PCR Plates into LIMS


 * 1) Bring the DNA plate along with your PCR plate to the barcode scanner in the lab.
 * 2) Go to LIMS http://131.104.104.75:8080/Lims.Web/
 * 3) Under Stage Selection choose PCR Prep.
 * 4) User Barcode: Scan in your card. Start plate: scan in the barcode on the extracted DNA.  End plate: put a barcode on your PCR plate and scan it in.
 * 5) Choose the Protocol you used (eg. If you used primers MLepF/LepR, that is what you choose from the list). Press Submit.  The plate barcodes will now be gone if it worked.
 * 6) Go back to the LIMS home page. Under Stage Selection choose PCR Thermocycling.
 * 7) User Barcode: Scan in your card. Plate barcode: scan in the barcode on your PCR plate.   Choose the Protocol (this is which program you ran the reaction on in the thermocycler, eg. COIFAST).
 * 8) Press Submit.

Return to PCR Procedure.

Continue to E-gel PCR Product Check.

Updated April 27 2009