FTA Cards


 * 1) When you receive FTA cards you have to punch out disks. A 1.2mm hole punch can be borrowed form Nataly at BIO.  You have to press the tip of the punch into the circle of paper that appears to have a sample on it and then press the button to release the disk of paper.
 * 2) Between samples it is important to punch a hole in filter paper.
 * 3) Wash two disks for one sample at the same time, in the same tube. Once a disk is washed it can be stored in the fridge up to one week before it is used.
 * 4) Add 200ul FTA buffer (it will probably be found on the counter) to the tube. Mix is occationally and wait 5 min.  Discard the liquid.  Repeat this step twice for a total of three washes.
 * 5) Add 200ul TE buffer (it should be found in the fridge) to the tube and let it sit for 5 min. Repeat this step once for a total of two washes.
 * 6) Use one disk in a 12.5ul PCR reaction. Make sure to add 2ul ddH2O and one disk rather than liquid DNA.

Next go to the PCR Procedure.

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Updated May 3 2009